Data for SEER-Map

This is Data for SEER-Map.
All the data (.nd2 files) are captured with NIS-Elements and can be open with NIS-Elements viewer.

Introduction

1. Hybridization optimization includes sample pretreatments in three conditions
2. 40-Round FISH includes the data of 40 Round hybridization-dissociation cycles.
3. Demo of Plant-microbiot image includes an in vivo SEER-Map analysis of a 30-strain microbial community colonizing the root of an Arabidopsis thaliana (Col-0) plant (Sample 1, Location 1).

4. Probe concentration titration includes the imaging data from a systematic titration experiment (0 nM to 200 nM) performed on a 7-member synthetic community  (related to Figure S1).

5. FISH Ctrl contains comprehensive specificity validation data (related to Figure S2).

6. Other plant samples includes imaging results demonstrating generalizability across diverse plant-microbe interfaces (related to Figure S3).