Dataset for the article “Ultrathin Electrospun Poly(γ-benzyl‑L‑glutamate) Nanofibrous Membranes for Retinal Pigment Epithelial Cell Cultivation”

Fig 1 a: SEM image of PBLG NfM; the inset shows the fiber diameter distribution measured from three different SEM images acquired from distinct regions of the membrane.

Fig 1 b: Schematic description indicating individual layers (left) and NfM fixed to a cell culture insert (right). 

Fig 1 c: WCA of PBLG NfM before air plasma treatment.

Fig 1 d: WCA of PBLG NfM after air plasma treatment.

Fig 1 e: SEM image of PBLG NfM PT FN.

Fig 2: Figure 2. High-resolution XPS spectra of the C 1s reference FN, PBLG NfM, PBLG NfM PT, PBLG NfM FN, and PBLG NfM PT FN. Measured spectra (thick black line) were deconvoluted with individual contributions (blue lines).

Fig 3: Normalized PL emission spectra of the FITC-FN (gray), PBLG NfM FITC-FN (blue), and PBLG NfM PT FITC-FN (red) samples measured with excitation at 488 nm.

Fig 4 a: Microscopic observations of pRPE cells on the PBLG NfM PT FN on day 7.

Fig 4 b: Microscopic observations of pRPE cells on the PBLG NfM PT FN on day 14, showing a more uniform RPE monolayer after 14 days compared to overpigmented clumps of RPE at 7 days.

Fig 7 a:  Live/dead cell assay of pRPE cultured on PBLG NfM PT FN. Dead cells.

Fig 7 b:   Live/dead cell assay of pRPE cultured on PBLG NfM PT FN. All cells.

Fig 7 c:  Live/dead cell assay of pRPE cultured on PBLG NfM PT FN. Merged.

Fig 8 a: Immunofluorescence staining for ZO-1 to assess tight junction formation, RPE phenotype, and nuclear morphology of RPE cells cultured on PBLG NfMs PT FN.

Fig 8 b: Immunofluorescence staining for PRE 65.

Fig 8 c: Immunofluorescence staining for DAPI.

Fig 8 d: Immunofluorescence staining merged.