eSRRF – Supplementary Data
eSRRF supplementary data The eSRRF preprint is available here: https://doi.org/10.1101/2022.04.07.487490 Find the code and documentation here: https://github.com/HenriquesLab/NanoJ-eSRRF 1a: Set01a_DNA-PAINT_COS7-MT.tif
eSRRF supplementary data
The eSRRF preprint is available here: https://doi.org/10.1101/2022.04.07.487490
Find the code and documentation here: https://github.com/HenriquesLab/NanoJ-eSRRF
1a: Set01a_DNA-PAINT_COS7-MT.tif
DNA-PAINT of fixed COS-7 cells with indirect immunolabeling against tubulin
- Pixelsize: 160 nm
- Exposure time: 30 ms
- 50 000 frames
1b: Set01b_DNA-PAINT_COS7-MT_bin100.tif
Dataset 1a with 100 frames binning to effectivly increase emitter density.
eSRRF analysis parameters:
- M=5,
- R=0.5,
- S=1
- all frames
2: Set02_HeLa_ffDronpa-MAP4.tif
Live-cell TIRF imaging dataset of HeLa cells expressing ffDronpa-MAP4
- Pixelsize: 156 nm
- Exposure time: 40 ms
eSRRF analysis parameters:
- M=4
- R=2
- S=4
- 100 frames
- rolling analysis gap = 50 frames
3: Set03_COS7_PrSS-mEmerald-KDEL.tif
Live-cell HiLO-TIRF of COS-7 cells expressing PrSS-mEmerald-KDEL marking the ER lumen.
- Pixelsize: 160 nm
- Exposure time: 5 ms
eSRRF analysis parameters:
- M=5
- R=2
- S=1
- 100 fr
- rolling analysis gap = 10 frames
4a: Set4a_MFM_HeLa_TOM20.tif
Live-cell MFM of HeLa cells expressing TOM20-HaloTag labeled with JF549
- Pixelsize: 160 nm
- Exposure time: 20 ms
eSRRF analysis parameters:
- M=4
- R=2
- S=1
- 100 fr
- rolling analysis gap = 25 frames
- 3D axial offset = 390 nm
4b: Set4b_MFM_beadcalibration.tif
MFM calibration data with TetraSpeck beads immobilized on a coverslip,
- Pixelsize: 160 nm
- z-step 60 nm
5: Set5_LLS_Jurkat_ER.zip
Lattice light sheet (LLS) data of the ER in Jurkat cells. The zip-folder contains single image stacks for each z-plane:
98 z-planes with 100 fr per plane at 10 ms exposure time 276 nm step size in the z-axis and 104 nm pixelsize.
Each z-plane stack was process with eSRRF followed by deskewing with the LLSM plugin (https://monash-merc.github.io/llsm/docs/fiji/index.html) to recover a single 3D volume reconstruction.
eSRRF analysis parameters:
- M=5
- R=3.5
- S=2
- 100 frames
6: Set6_TIRF_NeuronCulture_MT_Skylan-NS.tif
TIRF imaging data of cultured neuron expressing Skylan-NS tagged tubulin.
- Pixelsize: 64.7 nm
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Files are hosted on the source repository. Click download to access the full dataset.