mRNA-based influenza vaccine expands the breadth of the B cell response in humans

These are the processed BCR repertoire and transcriptomics data described in Matz et al., Nature Immunology, 2026. The raw sequencing data are available on SRA under BioProject PRJNA1049392.

Code

Code along with Docker containers for reproducing the NGS data-based figures and analyses in the published paper can be found on GitHub.

Metadata

File: WU397_matz_et_al_nat_imm_2026_meta.tsv

Abbreviations:

• LN = lymph node
• PB = plasmablast
• NS = no sorting

Processed BCR data – heavy chains

File: WU397_matz_et_al_nat_imm_2026_bcr_heavy.tsv.gz

Notes on columns:

The columns largely follow the AIRR-C Rearrangement format. The main deviation is that CDR3s were used, as opposed to IMGT-defined “junctions”. Nonetheless, junction-related columns are included here as some repositories use these. Non-standard columns are noted below.

• `cell_id`:  single-cell sequences follow the format `[donor]_[sample]@[id]`. 
• `sequence_id`: Sequence IDs follow the format `[donor]_[sample]@[id]`.
• `v_call_genotyped`: V gene annotation reassigned after individualized genotyping by TIgGER.
• `germline_[vdj]_call`: Clonal consensus germline calls after corresponding clonal consensus sequences were reconstructed via `CreateGermlines.py –cloned` from Change-O.
• `c_call`: constant gene annotation extracted from output of `cellranger vdj` for 10x sequences. Unlike `isotype`, `c_call` is down to the resolution of isotype subclass.
• `gex_anno`: Cell type identity annotation based on transcriptomic profiles. Mapped from `anno_leiden_0.95` from WU397_matz_et_al_nat_imm_2026_gex_b_cells.h5ad.
• `compartment`: B cell compartment.
• `timepoint` and `timepoint_2`: Timepoints in days. The latter simplifies `d180` and `d181` both as `d180`.
• `clone_id`: B cell clonal lineage IDs follow the format `[donor]@[id]`.
• `expressed`: `TRUE` if expressed as mAb; otherwise `FALSE`.
• `expressed_id`: IDs of expressed mAbs; `””` (blank) if not expressed.
• `ha_binding_binary`: Binary ELISA results for binding of recombinant mAbs to HA proteins. For expressed mAbs, `pos` and `neg` for positive and negative binding results respectively. `NA` if not expressed.
• `ha_binding
  

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