Sachs: Protein and Phospholipids Expressions
The Sachs dataset measures the expression level of different proteins and phospholipids in human cells. It includes the simultaneous measurements of 11 phosphorylated proteins and phospholipids derived from thousands of individual primary immune system cells, subjected to both general and specifi
The Sachs dataset measures the expression level of different proteins and phospholipids in human cells. It includes the simultaneous measurements of 11 phosphorylated proteins and phospholipids derived from thousands of individual primary immune system cells, subjected to both general and specific molecular interventions.
Task: The dataset can be used to study causal discovery algorithms.
Summary:
- Size of collection: 14 datasets on 11 features and 707 to 927 observations
- Task: Causal Discovery Problem
- Data Type: Continuous Data
- Dataset Scope: Collection of Datasets
- Ground Truth: Known Graph
- Temporal Structure: Static Data
- License: CC BY 4.0 (granted by G. Nolan and D. Lauffenburger)
- Missing Values: No Missing Data
Missingness Statement: There are no missing values.
Features:
Each sample in each dataset consists of quantitative amounts of each of the following 11 phosphorylated molecules, simultaneously measured from single cells:
| Measured molecule | Antibody specificity |
|---|---|
| Raf | Phosphorylation at S259 |
| Erk1 and Erk2 | Phosphorylation at T202 and Y204 |
| P38 | Phosphorylation at T180 and Y182 |
| Jnk | Phosphorylation at T183 and Y185 |
| Akt. | Phosphorylation at S473 |
| Mek 1 and Mek2 | Phosphorylation at S217 and S221 |
| PKA substrates | Detects proteins and peptides containing a phospho-Ser/Thr residue with arginine at the -3 position |
| PKC | Detects phosphorylated PKC-α, -βI, -βII, -δ, -ϵ, -η, and -θ isoforms only at C-terminal residue homologous to S660 of PKC-βII |
| Plcg | Phosphorylation at Y783 |
| PIP2 | Detects PIP2 |
| PIP3 | Detects PIP3 |
Files:
The datasets were collected after a series of stimulatory cues and inhibitory interventions, with cell reactions stopped at 15 minutes after stimulation by fixation, to profile the effects of each condition on the intracellular signaling networks of human primary naïve CD4+ T cells, downstream of CD3, CD28, and LFA-1 activation. The following conditions were used:
| File | Stimulation | Description |
|---|---|---|
| cd3cd28.csv | CD3, CD28 | T cell activation |
cd3cd28icam2.csv<
📤 Share this pageFound this useful? Share it with your network. Files are hosted on the source repository. Click download to access the full dataset. Mathematical Research Data Initiative (2023). Sachs: Protein and Phospholipids Expressions. https://doi.org/10.5281/zenodo.7681811
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