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A proposal for a standardised protocol to barcode all land plants

Mark W. Chase, Robyn S. Cowan, Peter M. Hollingsworth, Cássio van den Berg, Santiago Madriñán, Gitte Petersen, Ole Seberg, Tina Jørgsensen, Kenneth M. Cameron, Mark A. Carine, Niklas Pedersen, Terry A J Hedderson, Ferozah Conrad, Gerardo A. Salazar, James Edward Richardson, Michelle L. Hollingsworth, Timothy G. Barraclough, Laura J. Kelly, M. J. Wilkinson

📄 Abstract

Abstract We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. We review the other markers that have been proposed and discuss their advantages and disadvantages. The low levels of variation in plastid DNA make three regions necessary; there are no plastid regions, coding or non‐coding, that evolve as rapidly as mitochondrial DNA generally does in animals. We outline two, three‐region options, (1) rpoC1, rpoB and 1matK or (2) rpoC1, matK and psbA‐trnH as viable markers for land plant barcoding.

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