Analytical Strategies in Metabonomics

To perform metabonomics investigations, it is necessary to generate comprehensive metabolite profiles for complex samples such as biofluids and tissue/tissue extracts. Analytical technologies that can be used to achieve this aim are constantly evolving, and new developments are changing the way in which such profiles’ metabolite profiles can be generated. Here, the utility of various analytical techniques for global metabolite profiling, such as, e.g., 1 H NMR, MS, HPLC-MS, and GC-MS, are explored and compared. Keywords: global metabolite profiling • metabotyping • metabonomics • metabolomics • spectroscopic analysis • separations • hyphenation