Exosomes derived from platelet-rich plasma promote the re-epithelization of chronic cutaneous wounds via activation of YAP in a diabetic rat model

Chronic wounds have become an economic, social, and public health burden and need advanced treatment.Platelet-rich plasma (PRP) has been used extensively in treatment of chronic wounds because it contains an abundance of growth factors secreted by platelets.The exosomes derived from PRP (PRP-Exos) have been proven to encapsulate principal growth factors from platelets.This study is the first to show that these exosomes may exert the function of PRP.PRP-Exos can effectively induce proliferation and migration of endothelial cells and fibroblasts to improve angiogenesis and re-epithelialization in chronic wounds.We regulated YAP to verify the PRP-Exos-dependent effect on fibroblast proliferation and migration through YAP activation.In vivo, we observed the cutaneous healing process in chronic wounds treated with PRP-Exos in a diabetic rat model.We provide evidence of the probable molecular mechanisms underlying the PRP effect on healing of chronic ulcers and describe a promising resource of growth factors from exosomes without species restriction.