Plant Tissue and Cell Culture

Success in plant molecular biology and biotechnology is largely dependent on in vitro culture of plant cells and tissues.1-9 Transgenic crop technology offers an opportunity to scrutinize the in vivo function of particular gene products and to develop new cultivars having novel genetic traits. Genetically modi ed plants that contain foreign genes have been extensively developed using plant tissue culture techniques. Globally, around one billion ha of land was under cultivation of transgenic crops 35.1 Introduction …………………………………………………………………………………………………………..607 35.2 Concept of Totipotency in Plant Cell and Tissue Culture …………………………………………….608 35.3 Major Steps in Plant Cell and Tissue Culture Techniques ……………………………………………608