Test No. 236: Fish Embryo Acute Toxicity (FET) Test

© OECD, (2013) alcohol ( 9) has been missed in the FET.Therefore, if there are any indications that metabolites or other transformation products of relevance may be more toxic than the parent compound, it is also recommended to perform the test with these metabolites / transformation products and to also use these results when concluding on the toxicity of the test chemical, or alternatively perform another test which takes metabolism into further account. 7.For substances with a molecular weight ≥ 3kDa, a very bulky molecular structure, and substances causing delayed hatch which might preclude or reduce the post-hatch exposure, embryos are not expected to be sensitive because of limited bioavailability of the substance, and other toxicity tests might be more appropriate. VALIDITY OF THE TEST 8.For the test results to be valid, the following criteria apply: OECD/