Dataset for article: Safe and Natural Innovation in Regenerative Medicine: Enhancing Collagen Scaffold Stability with Vitamin B2 (Riboflavin)-Mediated UV Crosslinking

Dataset for article: Save and Natural Innovation in Regenerative Medicine: Enhancing Collagen Scaffold Stability with Vitamin B2 (Riboflavin)-Mediated UV Crosslinking contains data including in the article. 

Table 1: List of prepared samples and overview of associated crosslinking and processing parameters

Table 2: Comparison of tensile strength (UTC) and Young’s modulus (E) (mean ± standard deviation).

Figure 1: Schematic illustration of riboflavin-mediated collagen crosslinking under ultraviolet irradiation. (png)

Figure 2: CLSM analysis and visualization of porous collagen scaffolds: a) and c) crosslinked in 1 mM riboflavin and ethanol for 5 minutes; b) and d) crosslinked in 1 mM riboflavin and water for 5 minutes.

Figure 3:  SEM characterization of collagen scaffold morphology: (a) micrograph and pore size distribution histogram of the EDC/NHS-crosslinked scaffold used as a reference; (b) effect of riboflavin concentration (1, 10, 100, and 1000 µM; left to right) on scaffold morphology under UV-A and UV-C irradiation (15 min); (c) effect of UV irradiation time (5, 10, 15, and 30 min; left to right) on scaffold morphology under UV-A and UV-C irradiation (1000 µM riboflavin). All scale bars represent 200 µm. (jpg, excel table for pore size distribution)

Figure 4:  ATR-FTIR spectra of pure non-crosslinked collagen, EDC/NHS crosslinked scaffold, and riboflavin-UV crosslinked scaffolds, displaying the characteristic amide A, B, I, II, and III absorption bands. (unicode origin graph; to open it you need to have the origin available)

Figure 5: Second derivative of FTIR spectra of the amide I band for scaffolds crosslinked at 1000 μM riboflavin concentration with varying UV irradiation times (5; 10; 15; and 30 min.) using a) longwave UV-A radiation, and b) shortwave UV-C radiation. Second derivative of FTIR spectra of the amide I band for scaffolds crosslinked at 15 minutes UV irradiation with varying riboflavin concentrations (1; 10; 100; 1000 μM) using c) longwave UV-A radiation and d) shortwave UV-C radiation. (unicode origin graph – to open it you need to have the origin available ; excel for processing of  second derivative of FTIR spectra)

Figure 6: Swelling properties of collagen scaffolds a) crosslinked at 1000 μM riboflavin concentration with varying UV irradiation times b) crosslinked at 15 minutes UV irradiation with varying riboflavin concentrations. (excel data)

Figure 7: Degradation profiles of collagen scaffolds: a) and b) crosslinked at varying UV times; c) and d) crosslinked at varying riboflavin concentrations. (excel data)

Figure 8:  Viability of L929 fibroblast cells following exposure to extract media from scaffolds: